Bioinformatics Practice Test 6

1. Which of the following is incorrect regarding Differential In-Gel Electrophoresis?

Differentially expressed proteins in both conditions can’t be visualized in the same gel

Proteins from experimental and control samples are labeled with differently colored fluorescent dyes

In this, Differences in protein expression patterns can be detected in a similar way as in fluorescent-labeled DNA microarrays

Proteins are mixed together before electrophoresis on a two-dimensional gel

2. Which of the following is incorrect regarding the advantages of Molecular data for phylogenetics study?

They are more numerous than fossil records

More clear-cut and robust phylogenetic trees can be constructed with the molecular data

They are easier to obtain as compared to fossil records

Sampling bias is involved

3. ExPASY contains a number of programs to determine posttranslational modifications based on MS molecular mass data.

False

True

4. Which of the following is untrue about Lateral gene transfer?

There is exchange of genetic materials between species

It mainly occurs among prokaryotic organisms when foreign genes are acquired through mechanisms

It is one of the examples is transformation

It is also known as vertical gene transfer

5. A supervised analysis refers to classification of data into a set of predefined categories. For example, depending on the purpose of the experiment, the data can be classified into predefined ‘diseased’ or ‘normal’ categories.

False

True

6. Which of the following is untrue about the drawbacks of SAGE?

One or two sequencing errors in the tag sequence can lead to ambiguous or erroneous tag identification

Correctly sequenced SAGE tag sometimes may correspond to several genes or no gene at all

Correctly sequenced SAGE tag always corresponds to several genes

The drawback with this approach is the sensitivity to sequencing errors

7. For many eukaryotic proteins, newly synthesized protein precursors have to be transported to specific membrane-bound compartments and be proteolytically processed to become functional.

False

True

8. Which of the following is untrue regarding the Predicting Interactions Based on Domain Fusion?

A fused protein often reveals relationships between its domain components

A fused protein doesn’t necessarily reveal about the relationships between its domain components

It is based on gene fusion events

Predicting protein–protein interactions is called the “Rosetta stone” method

9. The justification behind Rosetta stone method is that when two domains are fused in a single protein, they have to be in _______ proximity to perform a common function.

extremely close

distant

extremely distant

10. The signal sequences have a _________ consensus but contain some specific features. They all have a ______ core region preceded by one or more positively charged residues.

weak, hydrophobic

weak, hydrophilic

strong, hydrophilic