Genetic Engineering Practice Test 7

1. Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

Double stranded circular molecule is used as a template

Single stranded circular molecule is used as a template

After amplification, mutation is introduced into one strand

Mutation is introduced into one of flanking primers

2. If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be?

complete

partial

carried out by a mixture of restriction enzymes

carried out by a different enzyme then that the restriction site

3. Down regulation of expression of endogenous genes by transformation with constructs that would generate sense RNA, rather than anti-sense RNA is known as ___________

anti-suppression

multisuppression

suppression

co-suppression

4. Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

self-complementary

double stranded

linear

circular

5. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

very less, is very less change

no, may be huge change

huge, may be no change

huge, would be huge change

6. Choose the incorrect statement for the method based on antisense RNA.

RNA is synthesized complementary to the sequence of the gene which is to be inactivated

It is achieved by placing a DNA sequence which encodes RNA complementary to the RNA to be inactivated

This method is preferred over gene disruption has gene inactivation can be achieved completely

Expression of the endogenous gene is diminished

7. Self cleavage reaction can take place in?

RNA

Can take place in both but is preferred in DNA

Both DNA and RNA

DNA

8. Isolation of RNA can be carried out easily from bacterial cells.

True

False

9. If methods are based on cellular processes that lead to inactivation of gene expression by affecting the RNA, then it is called as ___________

pre-transcriptional

translational

post-transcriptional

transcriptional

10. The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

may be activated or not

activated

destroyed

deactivated