Genetic Engineering Mock Test 2

10%

Question 1 of 10

1. Why internal secondary structures are not preferred for primers?

Because of it, primer may fold back on itself and won’t be available for template

Internal structures are very bulky and thus elongation is not preferred

If internal structures are present, no proof reading would be observed

Internal secondary structures require more amount of template

Question 1 of 10

Question 2 of 10

2. At times, a specific fragment of the molecules of DNA which are analyzed needs to be separated. One of the methods is digesting the gels simply. Which of the following statement is not correct in with respect to it?

For higher gelling temperatures, digestion of DNA is done via the addition of agarase or some chaotropic agents

The gelling temperature has an important role to play

For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting

The DNA can be extracted via the addition of sodium or any positively charged group

Question 2 of 10

Question 3 of 10

3. How can gradient in the field strength be obtained?

Buffer-gradient gels can be used with decreasing concentration of buffer at the bottom

Both can be varied, but resistance is having more effect

It can be obtained via varying the amount of resistance, keeping the current constant

It can be obtained via varying the amount of current, keeping the resistance constant

Question 3 of 10

Question 4 of 10

4. If the amount of agarose added is more, the molecular under analysis should have the following characteristic:

the amount of molecules under analysis matters

large size

size has no relation with the amount of agarose

small size

Question 4 of 10

Question 5 of 10

5. How many methods are there for obtaining the plasmid DNA from the bacteria?

Question 5 of 10

Question 6 of 10

6. Which of the following is useful in applications of PCR?

It is having a high speed

The amount of DNA required initially is high

It is manual

Only one sample’s analysis can be carried out at a time

Question 6 of 10

Question 7 of 10

7. Melting temperature is given by ____________

4(G+C) + 2(A+T)

4(A+G) + 2(C+T)

2(A+G) + 4(C+T)

2(G+C) + 4(A+T)

Question 7 of 10

Question 8 of 10

8. All the molecules generated during PCR will not be full length. Some will also be of intermediate length. Which of the statements is correct?

After first cycle, majority of the molecules will be full length and only some will be of intermediate length

The number of intermediate molecules increase geometrically and the number of target molecules increase arithmetically

The number of full length molecules increase as number of cycles proceed

In the next cycle, each intermediate molecule will generate one intermediate molecule and one target molecule

Question 8 of 10

Question 9 of 10

9. The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol.

False

True

Question 9 of 10

Question 10 of 10

10. Which of the following is a characteristic of Taq polymerase?

It is obtained from thermophilic bacterium and can be grown in the laboratory below a temperature of 75 degrees

It is heat stable

It is an RNA polymerase

It is used in cellular synthesis processes and the optimum temperature is at least 90 degrees

Question 10 of 10