Genetic Engineering Practice Test 3

1. How can the specificity of primer annealing be increased?

Adjusting the concentration of sodium ions

Use of short primers

Using polymerase with proof reading activity

Raising temperature

2. Sickle cell anaemia is a genetic disorder. Which of the following doesn’t holds true for it?

It can be analysed by PCR

The mutation is in alpha globulin gene

The conventional approach took weeks for the whole analyses to be carried out

It destroys a restriction site

3. Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?

The error caused by sequence heterogeneity is evenly distributed on all the codon positions

The error caused because of polymerase is evenly distributed on all the positions in the codon

The error caused because of polymerase is biased for the first position in the codon

The error caused by sequence heterogeneity is mainly because of first position in the codon

4. Generation of recombinants by randomly cloning fragments of total DNA from an organism is called as __________

shotgun cloning

screening

genomic library

recombination

5. What are the possibilities which can occur until the temperature has reached for primer annealing?

Extension doesn’t starts until the appropriate temperature is reached

Extension may start even when the temperature is low

There are more specific products which are generated

At low temperature, there is specific annealing of primer taking place

6. Which of the statement is incorrect for anchored PCR?

Fragment the sample DNA and ligate it to known sequence

The known sequence is attached to the required region of amplification and then further used as a second priming site

Tails are added enzymatically to the region of known sequence

Anchored PCR is the modification in which only one piece of the sequence is known and thus one primer

7. The phenomenon of not allowing the recombinants to escape into the environment is termed as __________

inactivation

disablement

blocking

termination

8. Which of the statement holds for long-range PCR and in its relation?

It is PCR in which a mixture of enzymes is used to have larger products

It is the PCR in which longer templates are used

DNA polymerases which don’t have proof-reading activity give larger products

DNA polymerases’ processivity is not a measure to have larger products

9. PCR is useful in population genetics because at times it can be used to study genetics of bacteria that can’t be cultured axenically.

True

False

10. Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

Circular PCR

Inverse PCR

In-situ PCR

Non-conventional PCR