Genetic Engineering Practice Test 6

1. The polypeptides produced after addition of mRNA are analysed with antibodies. Choose the incorrect statement for this analysis.

Protein A-Sepharose binds to IgG antibodies

Along with simple antigen-antibody complex, a substrate is added for easy precipitation

Protein A-Sepharose is added

Antibodies are added to each reaction tube and precipitation is simply based on antigen-antibody reaction

2. If the gene to be screened belongs to prokaryotic species then there are chances that it is not expressed.

True

False

3. Any cDNA library would represent a fraction of RNA species of an organism.

True

False

4. If the library to be screened and the host belongs to different species, then it is called as ___________

heterologous selection

mixed selection

intraspecies selection

homologous selection

5. Choose the incorrect statement with respect to host used.

The host should be deficient in recombination

The host if having restriction enzymes, then the incoming DNA should not be methylated

The host must carry mutation for the gene of interest

The host should not contain restriction enzymes

6. If physical methods are used, tracer is amplified and is labelled with biotin.

True

False

7. In the case of ligand binding by the expressed protein, the library can be screened by ______________

using a specific DNA sequence which can bind to the protein encoded by the sequence of interest

immunochemically, by the use of antibodies

all of the mentioned

ligands if the sequence we are looking for encodes a protein specific to a ligand

8. At what temperatures the hybridization and washing of the DNA probes should be carried out?

At a temperature higher than the melting temperature

At 100 degrees

At a temperature lower than the melting temperature

At melting temperature

9. Sometimes a gene which we want to clone is present on a particular chromosome. For this purpose, the chromosome should be in which phase?

Prophase

Telophase

Metaphase

Anaphase

10. In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________

library

mini library

shelf

small library