Genetic Engineering Mock Test 1

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Question 1 of 10

1. After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________

The ends created are always double stranded

One end is single stranded and one end is double stranded

Either the ends are single stranded or they are double stranded

The ends created are always single stranded

Question 1 of 10

Question 2 of 10

2. The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated?

The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity

Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity

The smaller fragment is named as Klenow fragment and the intact molecule is called as Kornberg fragment

The smaller fragment is having C terminal and the larger fragment is having N terminal

Question 2 of 10

Question 3 of 10

3. The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true?

The ends created by both the enzymes are not-compatible

The ends created by DpnI are single stranded

The ends created by both the enzymes are compatible

The ends created by Sau3A are single stranded

Question 3 of 10

Question 4 of 10

4. How many categories of ligation reaction are there on the basis of ends created?

Question 4 of 10

Question 5 of 10

5. The extent of deletions can be manipulated by controlling which of them?

Time of incubation only

They both have a role to play in the extent of the deletion

Amount of nuclease added

The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time

Question 5 of 10

Question 6 of 10

6. If blunt ended ligations are to be carried out. Which of the following enzymes can be used?

Both of these enzymes act equally in carrying out blunt ended ligations

T4 DNA ligase

E. coli DNA ligase

None of them is able to carry out blunt ended ligations

Question 6 of 10

Question 7 of 10

7. Isocaudomers are defined as the enzymes which recognize different sequence but generate same ends. Which of the following pairs of enzymes can be termed as isocuadomers?

BamHI (G|GATCC) and Sau3A (|GATC)

DpnI (GA|TC) and BglII (A|GATCT)

XbaI (T|CTAGA) and BamHI (G|GATCC)

DpnI (GA|TC) and Sau3A (|GATC)

Question 7 of 10

Question 8 of 10

8. The specificity of an enzyme is affected by the concentration of buffer used. This phenomenon is termed as:

specificity elevation

star activity

concentration gradient effects

diamond activity

Question 8 of 10

Question 9 of 10

9. Which of the following statements is correct regarding S1 nuclease?

It acts on both types of strands

It acts on single stranded DNA

It is obtained from E. coli

It acts on double stranded DNA

Question 9 of 10

Question 10 of 10

10. F plasmid is often used in conjugation. The correct statement is?

The F plasmid encodes the factor which is transferred from one cell to another

The factor encoded by the F plasmid is called as Filamentous (F) factor

The bacteria must belong to same species to carry out the conjugation

It is transferred from one cell to another by filament

Question 10 of 10

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