Genetic Engineering Practice Test 4

1. Choose the correct statement for cII gene and its related function?

This promoter is responsible to decide whether the life cycle would be lysogenic or lytic

The transcription is extended to the region of cII gene and it is termed as late transcription

cII gene is responsible for other gene which is gene cIII

They are responsible for deactivating the promoter for repressor establishment

2. When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

forced cloning

correct cloning

orientation cloning

restricted cloning

3. Which of the statement doesn’t holds for phage display systems?

The inserted is expressed along with other protein coat genes

The filamentous phage is often used as phage display system

Coding sequences are inserted in one of the protein coating genes

The most common protein coding genes are gene II or gene VIII

4. Is it necessary to stop the relegation of the central portion before the stiffer fragment is ligated.

False

True

5. Induction of lysogen takes place because of __________

Low levels of both cI and cII gene

Low level of cI gene

Low levels of cII gene

High levels of both cI and cII gene

6. Choose the incorrect statement with respect to the collection of single stranded molecules.

An infected culture is set up and after a while the cells are removed by centrifugation

The phage is in the supernatant and is precipitated by addition of glycol and sodium chloride

The single stranded molecules sediment at the bottom

The phage pellet is resuspended and phenol is used for removal of protein

7. Gene V blocks the production of which gene?

Gene IV

Gene II

Gene VIII

Gene III

8. Choose the correct statement for Sanger’s method of sequencing.

Use of dideoxynucleotide phosphate (ddNTP) is made

They are also called as chain termination methods

Use of M13 vector is made for getting double stranded DNA

The use of M13 vectors is more preferred these days

9. The uniform layer of cells is known as lawn and the holes peppered from infected cells are termed as __________

plaques

colony

pothole

holes

10. Insertion vectors are a type of vectors. Choose the correct statement for this type of vector.

These are complex vectors

There is an upper limit on the size of the DNA to be packaged

DNA is inserted through many restriction sites

Lambda gt09 is an example of this type of vector