Genetic Engineering Practice Test 10

1. Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence.

2. It is beneficial to use retrovirus for genetic manipulation of cells in the culture.

3. In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.

4. Embryonic stem cells are also used for generation of transgenic organisms. They are obtained from _______ of a developing ________

5. LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.

6. Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only.

7. rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________

8. Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis.

9. Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________

10. Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following?