Genetic Engineering Mock Test 6

10%

Question 1 of 10

1. If translation and transcription are taking place together, the reaction is called as ______ and if they are carried out separately, it is called as _______

linked, coupled

coupled, linked

grouped, separate

grouped, individual

Question 1 of 10

Question 2 of 10

2. In ______ organisms, firstly the gene is looked into model organisms.

large sized

both eukaryotic and prokaryotic

prokaryotic

eukaryotic

Question 2 of 10

Question 3 of 10

3. What is done after RNA fractionation is carried out?

Translation is carried out with a high background

Each band is translated in vivo

Amino acid is not radioactively labelled

Translation is carried out in wheat gram or lysate of rabbit reticulocyte cells

Question 3 of 10

Question 4 of 10

4. The process of finding a particular member of the library which is having some defined properties is called as ___________

searching

locating

screening

narrowing

Question 4 of 10

Question 5 of 10

5. If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true?

Non radiolabelled amino acids are used

The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc

The extract in which the host DNA constructs are incubated is capable of transcription only

The extract in which the host DNA constructs are incubated is capable of translation only

Question 5 of 10

Question 6 of 10

6. GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

It stands for Green Fluorescent Protein

The absorbed blue light produces green light which can be detected further

The blue light is produced because of binding of sodium ions

It is obtained from a bio-luminescent jellyfish and produces protein aequorin which emits blue light

Question 6 of 10

Question 7 of 10

7. If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.

False

True

Question 7 of 10

Question 8 of 10

8. Which of the following is desirable?

Driver alone

Driver- driver hybrid

Driver- tracer hybrid

Tracer- tracer hybrid

Question 8 of 10

Question 9 of 10

9. The important parameters for precise annealing of the probe and the subsequent washing don’t include ___________

Proportion of only A

Size of the probe

Ionic concentration of hybridization buffer

Other agents which alter the base pairing

Question 9 of 10

Question 10 of 10

10. The ligand should bind to a single protein only for the screening process.

True

False

Question 10 of 10