Genetic Engineering Mock Test 4

10%

Question 1 of 10

1. ccdB gene is used at times. Choose the correct statement with respect to this gene.

If intramolecular ligation takes place this protein is released

It is often regarded as control of cell death and birth gene

It is a control of cell birth gene

It is activated in complex vectors to increase the fraction of recombinants

Question 1 of 10

Question 2 of 10

2. Insertion vectors are a type of vectors. Choose the correct statement for this type of vector.

These are complex vectors

There is an upper limit on the size of the DNA to be packaged

DNA is inserted through many restriction sites

Lambda gt09 is an example of this type of vector

Question 2 of 10

Question 3 of 10

3. Which of the phage genes are responsible for phage excision?

xis gene

both xis and int gene

recA protein

int gene

Question 3 of 10

Question 4 of 10

4. endA mutation is often induced in host molecules. Choose the correct statement.

It activates the gene for DNA-specific endonuclease

It enhances the yield of plasmid DNA preparations only

It enhances both the quality and yield of plasmid DNA preparations

It enhances the quality of plasmid DNA preparations only

Question 4 of 10

Question 5 of 10

5. What happens if insert DNA is cut with two different restriction enzymes at the ends?

The amount of product increases

The insert can be ligated in one orientation only

It is difficult to insert the fragment

The insert can be ligated in any orientation

Question 5 of 10

Question 6 of 10

6. Choose the correct statement for the synthesis of RNA probe?

Probes are prepared for RNA transcripts which are specific for only particular strand

Double stranded DNA is only used for probe synthesis

Single stranded DNA can be used for probe synthesis

Probes are prepared for RNA transcripts which are specific for both the strands

Question 6 of 10

Question 7 of 10

7. Choose the incorrect statement with respect to rolling circle replication.

Without the cos sites, the packaging won’t be possible

It is for a single replication fork and yields a concatemeric molecule

The cleavage generates 10 nucleotide long overhangs

Staggered cleavage of DNA takes place at the cos sites

Question 7 of 10

Question 8 of 10

8. The fragment inserted in the place of the central portion of the genome is known as __________

insertion fragment

stuffer fragment

substitution fragment

displacement fragment

Question 8 of 10

Question 9 of 10

9. lacZΔM15 represents __________

it is partially deleted lacZ gene which is needed for alpha complementation

fully present lacZ gene

it represents the lacZ gene with M15 mutation

it is not required in alpha complementation

Question 9 of 10

Question 10 of 10

10. M13-plasmid vectors are often used. Which of the following is not a feature for this?

They are known as phagemid, plage or phasmid

Replication from M13 requires proteins to be provided by helper phage

Replication takes place through the origin of replication of M13 derivatives only

Some of the examples of these types of the combination are pUC 118, 119 and 120

Question 10 of 10