Genetic Engineering Mock Test 7

10%

Question 1 of 10

1. Choose the correct statement.

There are two flanking primers and they are having mutations

There is one primer which anneals the target sequence and is having mutation

One of the flanking primer and the primer annealing to the target sequence is having mutation

Either of the flanking primers or the primer is annealing to the target sequence is having mutation

Question 1 of 10

Question 2 of 10

2. Introduction into host organism can also be done by using a DNA construct, which when transcribed, generates an RNA which is _________

circular

linear

double stranded

self-complementary

Question 2 of 10

Question 3 of 10

3. The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene.

True

False

Question 3 of 10

Question 4 of 10

4. In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

Sulphur

Magnesium

Sodium

Calcium

Question 4 of 10

Question 5 of 10

5. Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as ___________

RNA vectors

Bacteriophage vectors

Cloning vectors

Expression vectors

Question 5 of 10

Question 6 of 10

6. The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

destroyed

may be activated or not

deactivated

activated

Question 6 of 10

Question 7 of 10

7. If a functional gene is disrupted while disrupting a restriction site _______ is created.

any other kind of mutation

either of the mutations

point mutation

frameshift mutation

Question 7 of 10

Question 8 of 10

8. The principle of gene disruption is ________ to replace the endogenous chromosomal copy of a gene with __________

reciprocal translocation, activated gene

reciprocal translocation, inactivated gene

homologous recombination, activated gene

homologous recombination, inactivated gene

Question 8 of 10

Question 9 of 10

9. The minor change in amino acid sequence can lead to _______ effect on three dimensional structure and there _____ in the primary sequence.

very less, is very less change

huge, may be no change

huge, would be huge change

no, may be huge change

Question 9 of 10

Question 10 of 10

10. It is easier to subclone a restriction fragment if it belongs to?

eukaryotic organism

large gene

prokaryotic organism

small gene

Question 10 of 10