Genetic Engineering Practice Test 5

1. If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as:

2. The first primer in the case of 3’ RACE is ___________

3. The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA.

4. Libraries using phage cloning vectors are often kept as ___________

5. What happens if chain-termination mutation is in the S gene?

6. Choose the incorrect statement for oligo-dT cellulose.

7. Selection of molecules by lack of inserts on the basis of the sacB gene is known as:

8. Chain-termination mutations in coat proteins genes are carried out. The coat protein coat genes used are __________

9. Which of the following doesn’t takes place after replication?

10. How many origins of replication are there once the cyclization is carried out by loxP sites?