Enzyme Technology Practice Test 6

1. _____________ may be obtained from immobilizing Escherichia coli on a cyanogen bromide-activated Sephadex G200 support.

Aminoacyclase

Lactase

Raffinase

Penicillin amidases

2. What does ‘Y’ represent in the following reaction?
HCN + 2H²O \(\underset{Y}{\rightarrow}\) HCOO^- + NH₄⁺

L-aspartic acid

Penicillin

Nitrilase

Cyanidase

3. In calorimetric biosensor, the temperature changes are measured by means of _________

Colorimetric test strips

Thermistors

Clark oxygen electrode

Ion-selective electrodes

4. Which of the following property is not possessed by biosensors?

The probe used in the biosensor must be tiny and biocompatible, having no toxic or antigenic effects

The biosensor should be cheap, small, portable and capable of being used by semi-skilled operators

The specificity of substrate binding is mediated by spatial arrangement of atoms in an enzyme’s substrate binding site as well as substrate.

There should be a market for the biosensor

5. Calorimetric biosensors represent the most generally applicable type of biosensor.

False

True

6. D-aminoacids are used in the production of _________

insecticides

glucose syrups

sunscreen agents

low calorific sweetener

7. Nitrilase is obtained from __________

Escherichia coli

Mortierella vinacea

Rhodococcus

Yeast

8. Which of the following biosensors function by the production of a current when a potential is applied between two electrodes?

Potentiometric biosensor

Amperometric biosensor

Calorimetric biosensor

Optical biosensor

9. Which of the following is not true for immobilized lactases?

Product inhibition by galactose

Microbial contamination

Unwanted oligosaccharide formation

Batch variability problems of acid hydrolysis

10. Which of these properties is not true for mediators?

The reduced form of the mediator should not readily react with oxygen

Mediators must be sufficiently soluble in oxidized and reduced forms

Increasing the heat output

Mediators must react rapidly with a reduced form of enzyme