Enzyme Technology Online Test

1. Which of the following property is not shown by isoenzyme?

2. In ________ method, the enzyme molecule in aqueous solution is confined within a semipermeable membrane.

3. Which one if the chemicals is not used to isolate enzymes from cell free extract?

4. In the following plot, what does X represent?

5. The immobilization method in which the enzyme is linked to the carrier matrix by covalent bond between them is referred to as __________

6. Which of the following methods cannot be used to isolate enzymes from cell free extract?

7. Which of the following is true for radioisotope method?

8. Calorimetric biosensors represent the most generally applicable type of biosensor.

9. Which of the following factor has a negative impact on substrate collision theory?

10. Which of the following lactase is used?

11. Which of the following technique separates the sample by percolating it through a bed of porous, uncharged particles?

12. Which of the following enzyme is not used in the brewing industry?

13. Which of the following chemical is not used to isolate enzymes from cell free extract?

14. Which of the following method can be applied when substrates molecules are much smaller than enzyme?

15. Group A: Substances are acceptable for use in food:: Group B: ____________

16. What is represented in the following diagram?

17. Which of the following is not Generally Regarded as Safe (GRAS) by the FDA, USA?

18. For positively charged substrates, \(K_m^{app}\) is given by which of the following?

19. Which of this is a disadvantage of aqueous 2 phase system?

20. Which of the following micro-organisms do not produce lactase?

21. Which of these is of primary importance to the enzyme producer and customer?

22. The following rate equation is given by ___________ inhibition.
\(V=\frac{\left (\frac{V_{max}}{1+\frac{[I]}{K_i'}}\right ) [S]}{\left (\frac{K_m}{1+\frac{[I]}{K_i'}}\right )+[S]}\)

23. Immobilized Rhodococcus is used at 10˚C and pH 8.0-8.5 in a semi-batchwise process with low acrylonitrile concentration to produce __________

24. Which of the following belongs to the class of isomerases?

25. Alanine transaminase is present in _________

26. Which of the following synzyme has 27% of the activity of a-chymotrypsin against 4-nitrophenyl esters?

27. Pick odd one out.

28. In defense, biosensors are used to sense taste, freshness, odor and to find out toxic substances.

29. _________ is used in treatment of skin ulcers.

30. Effectors that inhibit enzyme activity are termed as ____________

31. The following rate equation represents _____________ inhibition.
\(V=\frac{V_{max} [S]}{K_m (1+\frac{I}{K_i})+[S]}\)

32. Light beers of low calorific value have become popular in recent times.

33. Which of the following is used in myocardial infarction to dissolve clots?

34. Which of the following enzyme is used as a label?

35. Substances which reduce the rate of enzyme catalyzed reactions are known as ____________

36. In this below equation, Enzyme Y belongs to which class of enzymes?
A-X + H₂O \(_{\overrightarrow{Enzyme \,Y}}\) X-OH + AH

37. An increase in serum level of LDH₁ relative to LDH₂ is an indication of ___________

38. A method for the synthesis of aspartame exemplifies the power of thermolysin, a protease.

39. Which of the following enzyme is assayed and measured using spectrofluorometric method?

40. Which of these is not an ion-selective electrode?

41. What does ‘X’ represent in the following graph?

42. L-aspartic acid is used in _________ industry.

43. S-shaped curve may be shifted to the left by immobilizing the enzyme within highly hydrophilic support.

44. ___________ is defined as the enzyme’s binding efficiency with its appropriate substrate through optimized multiple non-covalent interactions.

45. The following reaction occurs at __________
4Ag⁰ + 4Cl^- → 4AgCl + 4e^-

46. The disruption of interactions between substrate and solvent is referred to as __________

47. In FPLC, the sample separates into various zones of sample components and is referred to as ________

48. Which of the following does not increase creatine kinase level?

49. What does the following equation represents?
ϕ=\(\frac{kPA_f}{ηD_f}\)

50. What reaction is represented in the diagram?