Enzyme Technology Mock Test 2

10%

Question 1 of 10

1. Pharmaceutical industry employs _____________ in order to recycle flow or add value to later products.

chromatography

plate and frame press

filtration

ultrafiltration

Question 1 of 10

Question 2 of 10

2. The solvent and other dissolve compounds that pass through the membrane is called __________

permeate

diffusate

retentate

filter cloth

Question 2 of 10

Question 3 of 10

3. What is the next step after organic solvent fractionation with dry ice - ethanol - water mixture?

Precipitated protein dissolved in 0.01 to 0.05 M cold buffer

Drying in freezer

Supernatant fractionation with ethanol

Refrigerated centrifugation at 3000 g for 15 mins

Question 3 of 10

Question 4 of 10

4. ___________ is responsible for maintaining biologically native structure of an enzyme and even nucleic acid.

Electrons

ATP

Proton

Question 4 of 10

Question 5 of 10

5. What is retentate?

Small molecules

Solvent outside

Solution is being dialyzed

Membrane

Question 5 of 10

Question 6 of 10

6. Which one of the following is not an example for ionic-detergent?

Cetyl trimethyl ammonium bromide

Sodium lauryl sulphate (SLS)

Sodium dodecyl sulphate (SDS)

Tween

Question 6 of 10

Question 7 of 10

7. The presence of enzyme in the sample can be detected by _________________

affinity chromatography

thin layer chromatography (TLC)

gel exclusion chromatography

ion exchange chromatography

Question 7 of 10

Question 8 of 10

8. Which of the following factors does not limit the rate of dialysis?

Type of substance

Diffusible substance concentration outside the membrane

Retentate volume to diffusate volume

Dialysis membrane relative surface area

Question 8 of 10

Question 9 of 10

9. Which procedure is represented in the following diagram?

Ammonium sulphate fractionation

Ammonium sulphate removal by dialysis

Ultrasonication

Homogenization.

Question 9 of 10

Question 10 of 10

10. Which of the following chemical is not used to isolate enzymes from cell free extract?

Polyethyleneimine

Magnesium chloride

Polyvinylpyrrolidone

EDTA

Question 10 of 10