Enzyme Technology Mock Test 10

10%

Question 1 of 10

1. The lab methods for measuring enzymatic activity for the study of enzyme kinetic and enzyme inhibition is referred to as _________

biosensors

enzyme assay

enzyme reactors

entrapment

Question 1 of 10

Question 2 of 10

2. Which of the following is not true for spectrofluorometric method?

Hydrolases can be assayed and measured by using this method

It is based on stoke shift

Trace impurities in enzyme can stop the emitted radiation

The intensity of emitted light is used to study enzyme reaction

Question 2 of 10

Question 3 of 10

3. Which of the following statement is not true for catalysis by approximation?

To make reactants proximal to each other

Change in conformation 3D structure of protein from low activity form to high activity form

This proximity raises effective concentration over that of the reactants free in solution and lead to rate acceleration

To quantify some proximity effects, model studies were carried out

Question 3 of 10

Question 4 of 10

4. In which of the following methods, the intensity of emitted light is used to study enzyme reaction?

Luminescence method

Biosensors

Spectrophotometer

Discontinuous assay

Question 4 of 10

Question 5 of 10

5. Which of these factors is true for enzymes while controlling assays?

pH (6-8) is suitable for maximum activity

Decrease in substrate concentration leads to increase in rate of reaction

Decrease in temperature leads to decreased reaction rates

Extreme high salt concentration can be tolerated

Question 5 of 10

Question 6 of 10

6. Proteolytic digestive enzymes which hydrolyze the peptide bond from the ends are referred to as _________

transaminase

exopeptidases

proteinases

endopeptidases

Question 6 of 10

Question 7 of 10

7. In which of the following assays, there is a “stop and sample” procedure?

Enzymatic assay technique

Discontinuous assay

Continuous assay

Coupled assay

Question 7 of 10

Question 8 of 10

8. Which of the following is not true for acid base catalysis?

Bases increases the reaction rate by increasing the nucleophilic character of the attacking group

To make reactants proximal to each other

Specific hydroxide ion catalysis of reaction in water is an example of this type of catalysis

Bases catalyze the reaction by accepting a proton

Question 8 of 10

Question 9 of 10

9. Which of these factors is not true for enzymes while controlling assays?

Extreme high salt concentration cannot be tolerated

pH (6-8) is suitable for maximum activity

Increase in substrate concentration leads to increase in rate of reaction

Decrease in temperature leads to decreased reaction rates

Question 9 of 10

Question 10 of 10

10. Which of the following statements are not true for gout disease?

Disease of joints

Blocking secretion of pancreatic juice

Kidneys are also affected

Joints become inflamed, painful and arthritic

Question 10 of 10