Enzyme Technology Practice Test 3

1. _____________ retains analyte molecules based on the coulombic interaction.

2. The process of determining the unknown concentration of a compound in a known solution by using HPLC is referred to as _______________

3. Which of this is not true for the active site of an enzyme?

4. In lock and key model, the enzyme catalyzed reaction may produce an adverse reaction.

5. The matrix selected during affinity chromatography must possess certain chemical groups.

6. The first step in preparation of affinity chromatography column is _____________

7. Acetylcholine esterase can be purified by using affinity chromatography with the use of _______ as a ligand.

8. A type of catalysis which involves covalent intermediate transition state formation between the cationic electrophilic group in the enzyme and the electron rich portion of the substrate is referred to as ____________

9. The energy required to attain transition state is referred to as _____________

10. Which of the following is not a highly specific biological interaction to be used in affinity chromatography?