Protein Engineering Practice Test 8

1. Which of the following is not true for error-prone PCR?

2. Which of the following is not a method to stabilize a protein?

3. The amount of enzyme produced by a micro-organism is not increased by increasing the number of gene copies that code for it.

4. The fragmentation of DNA in the DNA shuffling technique occurs at specific sites in DNA.

5. Which of the following is not a constituent enzyme of the pectinase group of enzymes?

6. Which of the following strategy of protein engineering is based on high throughput protein engineering?

7. Alignment of sequences of homologous enzymes with different specificities as well as structural information has proven useful as a guide to identifying residues possibly involved in the enzyme-substrate specificity.

8. All enzymes isolated thus far are specific for the type of chemical reaction they catalyze.

9. What is the temperature of the reaction mixture in the extension/elongation step of PCR?

10. If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?