Protein Engineering Mock Test 5

1. Which of the following cannot be used as a MALDI matrix compound?

2. Which of the following type of mass analyzer uses a magnetic field and cyclotron motion to trap ions?

3. What is the average mass of glycine?
Given data: Average mass of Carbon (C)=12.011,
The average mass of Hydrogen (H)=1.008,
The average mass of Oxygen (O)=15.999,
The average mass of Nitrogen (N)=14.007.

4. Who is the inventor of ESI-MS?

5. Which of the following ionization techniques involves the production of ions by spraying a solution of the analyte into an electric field?

6. Which of the following motif contains an amphipathic alpha-helix with a series of hydrophobic amino acid residues on one side?

7. Which of the following charge has the lowest m/z ratio?
Given data: q₁ has a charge +1 and mass of 800 Da,
q₂ has a charge +3 and mass of 3900 Da,
q₃ has a charge +2 and mass of 2970 Da,
q₄ has a charge +4 and a mass of 3480 Da.

8. What is the monoisotopic mass of proline?
Given data: Mass of the most abundant isotope of Carbon (C°)=12.0000,
Mass of most abundant isotope of Hydrogen (H°)=1.0078,
Mass of most abundant isotope of Oxygen (O°)=15.9949,
Mass of most abundant isotope of Nitrogen (N°)=14.0031.

9. ESI and MALDI belong to which of the following techniques?

10. Consider four ions q₁, q₂, q₃ and q₄. q₁ has a charge +3 and mass of 290 Da, q₂ has a charge +2, and mass of 290 Da, q₃ has a charge +3 and mass of 390 Da, and q₄ has a charge +2 and mass of 390 Da. Which one of these ions has a maximum m/z ratio?