Protein Engineering Mock Test 8

10%

Question 1 of 10

1. Which of the following is not a method of directed evolution?

DNA shuffling

Natural selection

Error-prone PCR

Phage display

Question 1 of 10

Question 2 of 10

2. Which of the following is the number of units of enzyme activity per milligram of total protein present?

International unit

Turnover number

Katal

Specific activity

Question 2 of 10

Question 3 of 10

3. Which of the following is not supposed to be present in the PCR reaction mixture solution?

Deoxynucleotide triphosphates (dNTPs)

Calcium ions

Buffer

Magnesium ions

Question 3 of 10

Question 4 of 10

4. What is the temperature of the reaction mixture in the second (annealing) step of PCR?

70-72°C

50-65°C

100-110°C

90-94°C

Question 4 of 10

Question 5 of 10

5. Which of the following is not a step of the DNA shuffling technique?

Phage display

Polymerase extension

Denaturation

Annealing

Question 5 of 10

Question 6 of 10

6. Which of the following is essential to keep separate the many pathways involving hundreds of enzymes, that function during metabolism?

Enzyme purity

Enzyme concentration

Enzyme quantity

Enzyme specificity

Question 6 of 10

Question 7 of 10

7. Which of the following is concerned with stability to pH?

Leather

Beer

Detergents

Food

Question 7 of 10

Question 8 of 10

8. Which of the following is not true for DNA shuffling?

It involves random DNA fragmentation

It involves the addition of primers

It involves many cycles of PCR

It recombines beneficial mutations from different molecules

Question 8 of 10

Question 9 of 10

9. In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection.

True

False

Question 9 of 10

Question 10 of 10

10. Which of the following is not a property of enzymes?

Catalytic power

Specificity

Acts as ampholytes

Irreversibility

Question 10 of 10