Protein Engineering Mock Test 10

10%

Question 1 of 10

1. The C-terminal domain of tyrosyl-tRNA synthetase contains major binding determinants for the tRNA.

False

True

Question 1 of 10

Question 2 of 10

2. Elimination of disulfide bond between which two amino acid residues within the A3 domain increases FVIII secretion by 2-fold?

C1785 and C1798

C1899 and C1903

C1414 and C1456

C1123 and C1158

Question 2 of 10

Question 3 of 10

3. Strep-tag II hampers protein folding or secretion and it usually interferes with protein function.

True

False

Question 3 of 10

Question 4 of 10

4. Which of the following enzyme catalyzes the charging of tRNA^Tyr with tyrosine?

Tyrosine synthetase

Tyrosyl-tRNA synthase

RNA activating enzyme

Tyrosyl-tRNA synthetase

Question 4 of 10

Question 5 of 10

5. Both the subunits of heterodimers are involved in tRNA binding.

True

False

Question 5 of 10

Question 6 of 10

6. The affinity of Strep-tag for streptavidin is very high when it is placed at the amino terminus or in between two protein domains.

True

False

Question 6 of 10

Question 7 of 10

7. Neutralizing antibodies do not abrogate viral infectivity by which of the following mechanisms?

Inducing the formation of non-infectious viral aggregates

Degrading the virus directly

Blocking virus attachment to host cells

Inhibiting viral uncoating

Question 7 of 10

Question 8 of 10

8. In tRNA charging, the K_m (ATP) is unaffected in the heterodimer, but the K_cat is one half that of wild type enzyme.

False

True

Question 8 of 10

Question 9 of 10

9. The FVIII heavy chain and light chain dissociate after cleavage by the furin.

False

True

Question 9 of 10

Question 10 of 10

10. Which of the following is not the way in which antibodies participate in host defense?

Enhance uptake by phagocytic cells

Opsonization

Activation of complement proteins

Directly destroy the antigen

Question 10 of 10