Cytogenetics Mock Test 3

10%

Question 1 of 10

1. Which of these factors can recognize the UAA stop codon?

RF1

RRF

Ef-G

RF3

Question 1 of 10

Question 2 of 10

2. In an experiment you grow some cells in a media containing heavy isotopes of C and N. Then you transfer then to a lighter media. If you centrifuge the ribosomes so obtained after an hour how many bands will you expect?

Question 2 of 10

Question 3 of 10

3. Puromycin is a ribosomal antibiotic. It binds to ________

16S rRNA

Peptide exit channel

A site of unassociated 16S rRNA

tRNA binding site

Question 3 of 10

Question 4 of 10

4. You gradually treat ribosome with SDS followed by Proteinase K and the finally extract it with phenol. Which of the options match the result you expect?

It stands no treatment at all

Ribosome withstands all these treatments and still has peptidyl transferase activity

It stands upto SDS treatment

It stands upto proteinase K treatment

Question 4 of 10

Question 5 of 10

5. Which factors lead to disassociation of the ribosomal subunits?

Rf1 and RRF

RRF and Ef-G

IF-1 and IF-3

IF1 and IF2

Question 5 of 10

Question 6 of 10

6. In an experiment you delete a part of the 5’ UTR of transferring mRNA, what will be its effect on iron sensitivity?

No change

It will be able to sense iron changes better

It will have leaky sensitivity

It will not be able to sense the iron changes

Question 6 of 10

Question 7 of 10

7. What is used to regulate translation initiation in eukaryotes?

Phosphorylation of IF2

Turnover of mRNA

Methylation of IF1a

Ubiquitination of ribosome

Question 7 of 10

Question 8 of 10

8. When the intracellular iron level is low, the turnover of transferrin mRNA is________

Kept normal

Increased

Doesn’t effect

Decreased

Question 8 of 10

Question 9 of 10

9. The 50S ribosomal subunit is folded in ______ major domains.

Question 9 of 10

Question 10 of 10

10. Which of the following factors prevent reassociation of the 40S and 60S subunits when not bound to the mRNA?

eIF1 and eIF3

eIF3 and eIF4

eIF3 and eIF6

eIF2 and eIF3

Question 10 of 10