Cytogenetics Mock Test 1

1. In case of eukaryotes replication initiates at ________

2. You grow a bacterial culture in a media containing N¹⁵ and transfer it to a media with N¹⁴. After two rounds of replication you perform a CsCl density gradient centrifugation of the DNA. How many bands will you observe what will be their intensity?

3. Pre-replication complex is necessary to initiate replication. It assembles in______________

4. In an experiment you wrongly added Rifampicin antibiotic to Rifampicin sensitive bacteria. You try to correct your mistake by adding more core subunits (resistant to Rifampicin) to your mixture. What would you expect to see?

5. Which protein mentioned below can reverse central dogma?

6. If you study T4 bacteriophage transcription with holoenzyme which genes will you expect to be transcribed?

7. When sigma subunit dissociates from the RNA polymerase what happens to the DNA binding clamp of the core?

8. Which of these operons is anabolic?

9. Which of these Ara genes is a mode of feedback autoregulation?

10. In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1^st set you deleted 94 amino acids from the C-terminus and in the 2^nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?