Cytogenetics Mock Test 2

1. In a substrate X catabolic gene promoter you replace the normal histones by your experimental histones – ones with arginine side chains in place of histidine. What will be the effect on the concentration of substrate X against the control?

2. In the case of group 1 RNA, which one of the following can’t attack?

3. In an experiment you add growing concentration of the alpha amanitin. What will be the sequence in which RNA polymerases are affected if at all?

4. You take a crude cell lysate and treat it with DNase. Now wash it and add protease. Use specific restriction endonuclease to get your desired fragment and run it on a gel. You see a single band. Which of the following will be true about the fragment?

5. PAP has a processivity of ___________

6. ESE bound to SR proteins ______________ splicing and ESE bound to hnRNA ______________ splicing.

7. U rich RNA that helps in splicing are______________

8. You isolate the nucleolus from the nucleus and performed a density gradient centrifugation with the extract. Then you check the different for the RNA polymerase activity of the different segment. Which RNA polymerase would you expect to find in abundance?

9. What is the sequence of the heptad repeat of C terminal domain?

10. The absence of which factor will prevent TFIIH and TFIIE recruitment?