Vector Biology and Gene Manipulation Practice Test 1

1. Which of the following is an example of flush end cutter?

2. What is the role of Rop protein in a plasmid?

3. The enzyme Bal31 purified from the bacterium Alteromonas Espejiana is an example of which enzyme?

4. Enzymes that remove nucleotides one at a time from the end of a DNA molecule are called ____________

5. What is the problem associated with adaptors?

6. lacZ’ gene present in a Puc series plasmid codes for which of the enzyme?

7. Col plasmids are responsible for ______

8. Where are the genes that encode for proteins required for replication encoded in a plasmid?

9. How can it be determined that a DNA molecule is cleaved by the action of endonuclease?

10. What are relaxed plasmids?