DNA Cloning Test - 2

1. DNA Ligase, used in recombinant DNA technology is obtained from _______________

Saccharomyces

E.coli and also Ligase encoded by T4 phage

Retroviruses

E.coli only

2. Which of the following is true about plasmids?

They are present in plants and animals

They are present in bacteria only

They are present in all organisms

They are present in bacteria and phages

3. Generally, YAC contains how many elements?

4. In hybridization method, which of the following is pressed onto agar?

PVDF

Nitrocellulose paper

Filter paper

Silicon paper

5. Where does a restriction endonuclease cuts DNA?

Replication site

Ligation site

Ori

Recognition sequence

6. Which of the following statements is true about a vector?

Fungi can also be used as vectors

All vectors are plasmids

Cyanobacteria can also be used as vectors

Plasmids, phages can be used as vectors

7. Which of the following temperature best suits for the growth and multiplication of transformed cells?

30°C

37°C

35°C

36°C

8. What is the main aim of human genome project?

To improve techniques of finger printing

To identify and sequence of all the genes present in the human body

To remove disease causing genes from humans

To introduce new genes to human beings

9. Arrange the following steps involved in Gene cloning in the correct order.

i) Isolation of desired gene
ii) Insertion of isolated gene to the vector
iii) Introduction of recombinant vector to the host
iv) Expression of recombinant gene in host
v) Extraction of recombinant gene product

i, iii, ii, iv, v

v, iv, iii, ii, i

iv, v, ii, i, iii

i, ii, iii, iv, v

10. Which of the following is a DNA element that instructs RNA polymerase where to bind on DNA?

Promoter

Enhancer

Hormone response element

Operator

11. Site directed mutagenesis facilitated research on __________

Proteins

Lipids

Carbohydrates

Fats

12. Recombinant DNA molecule is produced by ligating __________

Two m-RNA molecules

One m-RNA with one t-RNA

Two DNA segments

One m-RNA with DNA segment

13. Agar is extracted from ____________

Virus

Fungi

Bacteria

Algae

14. The work ‘Hybridization’ in DNA finger printing means ____________

Pairing between the nucleotides of probe with mRNA

Pairing between the nucleosides with mRNA

Pairing between the nucleotides of DNA and mRNA

Pairing between the nucleotides of DNA sample with probe

15. Which of the following endonuclease removes a length of DNA between two telomere sequences?

HindIII

EcoR2

BamHI

EcoR1